1RSS
RIBOSOMAL PROTEIN S7 FROM THERMUS THERMOPHILUS
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1997-05-15 |
| Detector | BRANDEIS |
| Wavelength(s) | 0.98, 1.01 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 55.530, 55.530, 103.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 1.900 |
| R-factor | 0.229 |
| Rwork | 0.229 |
| R-free | 0.28400 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 21.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.980 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.033 | 0.064 |
| Total number of observations | 103923 * | |
| Number of reflections | 13428 | |
| <I/σ(I)> | 37 | 5 |
| Completeness [%] | 99.8 | 95 |
| Redundancy | 7.7 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | 4-13 * | CRYSTALLIZED FROM 15% PEG 8000, 20% GLYCEROL, 6% MPD, 0.1 M NAHCO3, 0.1 M TRIS PH 8.0 AT 4 C, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | PEG8000 | ||
| 2 | 1 | drop | MPD | 2.5-5 (%) | |
| 3 | 1 | drop | protein | 3.5 (mg/ml) | |
| 4 | 1 | drop | HEPES | 2.5 (mM) | |
| 5 | 1 | drop | 0.05 (M) | ||
| 6 | 1 | drop | 2-mercaptoethanol | 2.5 (mM) | |
| 7 | 1 | reservoir | PEG8000 | ||
| 8 | 1 | reservoir | MPD | 5-10 (%) |
