1RSM
THE 2-ANGSTROMS RESOLUTION STRUCTURE OF A THERMOSTABLE RIBONUCLEASE A CHEMICALLY CROSS-LINKED BETWEEN LYSINE RESIDUES 7 AND 41
Experimental procedure
Spacegroup name | P 21 21 21 |
Unit cell lengths | 37.050, 41.260, 75.640 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 * - 2.000 |
R-factor | 0.184 * |
Rwork | 0.184 |
RMSD bond length | 0.024 |
RMSD bond angle | 0.038 * |
Refinement software | PROLSQ |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 2.100 * |
High resolution limit [Å] | 2.000 * |
Rmerge | 0.081 * |
Total number of observations | 10701 * |
Number of reflections | 8301 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microdialysis * | 8 * | 7 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ethanol | 30 (%(v/v)) | 50mM-Tris-HCl(pH7.5) and 50 mM-imidazole(pH8.0) are also suitable |