1RSG
Crystal structure of the polyamine oxidase Fms1 from yeast
Experimental procedure
Experimental method | SAD |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F2 |
Synchrotron site | CHESS |
Beamline | F2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-08-31 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.979106 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 162.385, 103.126, 77.590 |
Unit cell angles | 90.00, 94.98, 90.00 |
Refinement procedure
Resolution | 35.950 - 1.900 |
R-factor | 0.21 |
Rwork | 0.206 |
R-free | 0.24500 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SAPI |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.051 | 0.302 |
Number of reflections | 96862 | |
Completeness [%] | 96.7 | 73 |
Redundancy | 6.4 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | PEG 3350, Tris-HCl, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | PEG 3350, Tris-HCl, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |