1RSE
MYOGLOBIN (HORSE HEART) MUTANT WITH SER 92 REPLACED BY ASP (S92D)
Experimental procedure
Detector technology | IMAGE PLATE |
Collection date | 1993-05-28 |
Detector | RIGAKU |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 64.420, 28.880, 35.970 |
Unit cell angles | 90.00, 107.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 1.700 |
R-factor | 0.188 * |
Rwork | 0.188 |
RMSD bond length | 0.015 |
RMSD bond angle | 0.031 |
Data reduction software | OSCILL |
Refinement software | PROLSQ |
Data quality characteristics
Overall | |
Rmerge | 0.059 |
Total number of observations | 45864 * |
Number of reflections | 11967 |
Completeness [%] | 85.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.8 * | 21 * | used to seeding * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15 (mg/ml) | |
2 | 1 | drop | ammonium sulfate | 59 (%sat) | |
3 | 1 | drop | Tris-HCl | 20 (mM) | |
4 | 1 | drop | EDTA | 1 (mM) | |
5 | 1 | reservoir | ammonium sulfate | 67 (%sat) | |
6 | 1 | reservoir | Tris-HCl | 20 (mM) | |
7 | 1 | reservoir | EDTA | 1 (mM) |