1RSB
X-ray study of the DNA oligomer d(ATATAT) in P65 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-01-30 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.81 |
| Spacegroup name | P 65 |
| Unit cell lengths | 32.313, 32.313, 117.779 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 2.170 |
| Rwork | 0.218 |
| R-free | 0.26250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NDB entry UD0035 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.193 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.220 |
| High resolution limit [Å] | 2.170 | 2.170 |
| Rmerge | 0.046 | 0.460 |
| Number of reflections | 3693 | |
| <I/σ(I)> | 38.2 | 3.1 |
| Completeness [%] | 98.3 | 97.7 |
| Redundancy | 19.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 286 | Spermine, NaCl,MPD,cacodylate, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 286K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Spermine | ||
| 2 | 1 | 1 | NaCl | ||
| 3 | 1 | 1 | MPD | ||
| 4 | 1 | 1 | cacodylate | ||
| 5 | 1 | 1 | H2O | ||
| 6 | 1 | 2 | MPD | ||
| 7 | 1 | 2 | cacodylate | ||
| 8 | 1 | 2 | H2O |
