1RRO

REFINEMENT OF RECOMBINANT ONCOMODULIN AT 1.30 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	39.700, 65.120, 32.940
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.300
R-factor	0.176
RMSD bond length	0.024
RMSD bond angle	0.038 *
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	1.240 *
Rmerge	0.051 *
Total number of observations	115881 *
Number of reflections	22446 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.2 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	1.4 (%)
2	1	drop	cacodylate	5 (mM)
3	1	drop		10 (mM)
4	1	drop	dithiothreitol	0.5 (mM)
5	1	drop	PEG6000	11 (%)
6	1	reservoir	PEG6000	33 (%(w/v))
7	1	reservoir	cacodylate	10 (mM)
8	1	reservoir	dithiothreitol	1 (mM)