1RPK
Crystal structure of barley alpha-amylase isozyme 1 (amy1) in complex with acarbose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-11-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 93.390, 72.920, 61.390 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.360 - 2.000 |
| R-factor | 0.18 |
| Rwork | 0.180 |
| R-free | 0.23100 |
| Structure solution method | FOURIER DIFFERENCE |
| Starting model (for MR) | 1ht6 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.360 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 27344 | |
| <I/σ(I)> | 4.2 | 1.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.5 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 290 | PEG 8000, ISOPROPANOL, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
