1RPG

STRUCTURES OF RNASE A COMPLEXED WITH 3'-CMP AND D(CPA): ACTIVE SITE CONFORMATION AND CONSERVED WATER MOLECULES

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	30.000, 38.270, 53.170
Unit cell angles	90.00, 106.00, 90.00

Refinement procedure

Resolution	10.000 - 1.400
R-factor	0.172
RMSD bond length	0.017
RMSD bond angle	0.032
Refinement software	RESTRAIN

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000 *
High resolution limit [Å]	1.400 *
Rmerge	0.047 *
Total number of observations	92138 *
Number of reflections	20881 *
Completeness [%]	91.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	35 (mg/ml)
2	1	drop	sodium phosphate	20 (mM)
3	1	drop	sodium acetate	20 (mM)
4	1	reservoir	ammonium sulfate	35 (%)
5	1	reservoir	sodium chlorine	1.5 (M)