1RPF

THE STRUCTURES OF RNASE COMPLEXED WITH 3'-CMP AND D(CPA): ACTIVE SITE CONFORMATION AND CONSERVED WATER MOLECULES

Experimental procedure

Spacegroup name	P 32 2 1
Unit cell lengths	65.250, 65.250, 65.520
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.200
R-factor	0.155
RMSD bond length	0.018
RMSD bond angle	0.033
Refinement software	RESTRAIN

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000 *
High resolution limit [Å]	2.200 *
Rmerge	0.103 *
Total number of observations	43289 *
Number of reflections	8395 *
Completeness [%]	99.7 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	35 (mg/ml)
2	1	drop	sodium phosphate	20 (mM)
3	1	drop	sodium acetate	20 (mM)
4	1	reservoir	ammonium sulfate	35 (%)
5	1	reservoir	sodium chlorine	1.5 (M)