1RPA
THREE-DIMENSIONAL STRUCTURE OF RAT ACID PHOSPHATASE IN COMPLEX WITH L(+) TARTRATE
Experimental procedure
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 89.400, 89.400, 152.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 * - 3.000 |
| R-factor | 0.215 |
| Rwork | 0.215 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 4.000 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 3.000 * |
| Rmerge | 0.057 * |
| Total number of observations | 24008 * |
| Number of reflections | 11350 * |
| Completeness [%] | 80.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.4 * | 4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 8-10 (mg/ml) | |
| 2 | 1 | reservoir | sodium acetate | 0.1 (M) | |
| 3 | 1 | reservoir | PEG6000 | 12-15 (%) |
