1RP1
DOG PANCREATIC LIPASE RELATED PROTEIN 1
Experimental procedure
Source type | SYNCHROTRON |
Source details | LURE BEAMLINE DW32 |
Synchrotron site | LURE |
Beamline | DW32 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1997-09 |
Detector | MARRESEARCH |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 54.026, 150.748, 132.006 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 9.000 - 2.100 |
R-factor | 0.195 |
Rwork | 0.195 |
R-free | 0.25600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1thg |
RMSD bond length | 0.012 |
RMSD bond angle | 26.400 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.843) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 9.000 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.067 | 0.368 |
Number of reflections | 30017 | |
<I/σ(I)> | 11.2 | 3 |
Completeness [%] | 97.8 | 94.6 |
Redundancy | 3.4 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 4.2 | drop was diluted with an equal volume of the reservoir solution * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 9.5 (mg/ml) | |
2 | 1 | reservoir | PEG8000 | 20 (%) | |
3 | 1 | reservoir | potassium phosphate | 25 (mM) |