1ROW

Structure of SSP-19, an MSP-domain protein like family member in Caenorhabditis elegans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-10-24
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	52.354, 31.848, 55.577
Unit cell angles	90.00, 97.67, 90.00

Refinement procedure

Resolution	24.790 * - 2.200 *
R-factor	0.219 *
Rwork	0.225
R-free	0.28000 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Homology model (Swiss Model)based on PDB Entry 1M1S
RMSD bond length	0.007 *
RMSD bond angle	1.300 *
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.200 *	2.200 *
Rmerge	0.053 *	0.087 *
Total number of observations	28027 *
Number of reflections	8710 *	852 *
<I/σ(I)>	33.9
Completeness [%]	92.4 *	89.7 *
Redundancy	3.3	3.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	295	1.7M lithium sulfate, 0.05M Mes, 0.1M magnesium chloride, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7 (mg/ml)
2	1	drop	HEPES	20 (mg/ml)	pH7.5
3	1	drop	octyl beta-D-glucopyranoside	0.5 (%(v/v))
4	1	reservoir	lithium sulfate	1.7 (M)
5	1	reservoir	MES	50 (mM)	pH5.6
6	1	reservoir		100 (mM)