1ROM
CRYSTAL STRUCTURE OF NITRIC REDUCTASE FROM DENITRIFYING FUNGUS FUSARIUM OXYSPORUM
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 288 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-12-10 |
| Detector | FUJI |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.990, 82.660, 87.210 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.000 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| R-free | 0.26600 |
| Structure solution method | MIR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 22.800 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA) |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.240 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.054 | 0.107 |
| Total number of observations | 84152 * | |
| Number of reflections | 24733 | |
| <I/σ(I)> | 9.9 | 7 |
| Completeness [%] | 90.1 | 79.5 |
| Redundancy | 3.4 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 5.5 | Park, S.Y., (1997) FEBS Lett. 412, 346. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | MES | 100 (mM) | |
| 2 | 1 | drop | protein | 25 (mg/ml) | |
| 3 | 1 | drop | MES | 50 (mM) | |
| 4 | 1 | reservoir | PEG4000 | ||
| 5 | 1 | drop | PEG4000 |
