1RO8
Structural analysis of the sialyltransferase CstII from Campylobacter jejuni in complex with a substrate analogue, cytidine-5'-monophosphate
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-09-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97912, 0.97961, 0.97900 |
| Spacegroup name | P 4 |
| Unit cell lengths | 115.434, 115.434, 41.059 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 2.100* |
| R-factor | 0.258 |
| Rwork | 0.220 |
| R-free | 0.25600 * |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.170 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.180 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 62730 | |
| <I/σ(I)> | 23.9 | 10.6 |
| Completeness [%] | 95.0 | 95 |
| Redundancy | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | PEG 6000, MPD, Tris, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | 10 (mM) | ||
| 3 | 1 | drop | donor | 10 (mM) | |
| 4 | 1 | reservoir | HEPES | 100 (mM) | pH7.5 |
| 5 | 1 | reservoir | PEG6000 | 10 (%(w/v)) | |
| 6 | 1 | reservoir | MPD | 5 (%(v/v)) |
