1RO5
Crystal Structure of the AHL Synthase LasI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2001-12-11 |
| Detector | SBC-2 |
| Wavelength(s) | 1.033 |
| Spacegroup name | F 2 3 |
| Unit cell lengths | 154.900, 154.900, 154.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.580 - 2.300 |
| R-factor | 0.19712 |
| Rwork | 0.193 |
| R-free | 0.23637 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.036 |
| RMSD bond angle | 2.382 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 500.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.103 | 0.221 |
| Number of reflections | 13180 | |
| <I/σ(I)> | 25.14 | 4 |
| Completeness [%] | 95.0 | 77 |
| Redundancy | 7.52 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 303 | Ammonium Sulfate, Sodium Sulfate, MOPS, NaCl, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
