1RNZ

RIBONUCLEASE A CRYSTALLIZED FROM 2.5M SODIUM CHLORIDE, 3.3M SODIUM FORMATE

Experimental procedure

Source type	ROTATING ANODE
Temperature [K]	290
Detector technology	AREA DETECTOR
Collection date	1993
Detector	SIEMENS
Spacegroup name	P 32 2 1
Unit cell lengths	64.562, 64.562, 65.149
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 1.900
R-factor	0.167 *
Rwork	0.167
RMSD bond length	0.011
RMSD bond angle	0.030
Data reduction software	XDS
Data scaling software	XDS
Phasing software	X-PLOR
Refinement software	PROFFT

Data quality characteristics

	Overall
Low resolution limit [Å]	27.900
High resolution limit [Å]	1.900
Rmerge	0.049
Number of reflections	11350
<I/σ(I)>	17.04
Completeness [%]	89.0
Redundancy	2.19

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.5		CRYSTALLIZED FROM 2.5M SODIUM CHLORIDE, 3.3M SODIUM FORMATE, pH 5.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	50 (mg/ml)
2	1	reservoir	sodium formate	3.3 (M)
3	1	reservoir		2.5 (M)
4	1	reservoir	sodium acetate	0.1 (M)