1RNY

RIBONUCLEASE A CRYSTALLIZED FROM 3M CESIUM CHLORIDE, 30% AMMONIUM SULFATE

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	290
Detector technology	AREA DETECTOR
Collection date	1993
Detector	SIEMENS-NICOLET X100
Spacegroup name	P 32 2 1
Unit cell lengths	64.763, 64.763, 65.338
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.000
R-factor	0.175 *
Rwork	0.175
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	RIBONUCLEASE A
RMSD bond length	0.011 *
RMSD bond angle	2.620
Data reduction software	XDS
Data scaling software	XDS
Phasing software	X-PLOR
Refinement software	PROFFT

Data quality characteristics

	Overall
Low resolution limit [Å]	8.200
High resolution limit [Å]	2.000
Rmerge	0.044
Number of reflections	9388
<I/σ(I)>	21.91
Completeness [%]	84.5
Redundancy	2.34

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.5		CRYSTALLIZED FROM 80% AMMONIUM SULFATE (PDB ENTRY 1RNO), pH 5.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	50 (mg/ml)
2	1	reservoir	ammonium sulfate	30 (%)
3	1	reservoir		3.0 (M)
4	1	reservoir	sodium acetate	0.1 (M)