1RNW

RECOMBINANT RIBONUCLEASE A CRYSTALLIZED FROM 80% AMMONIUM SULPHATE

Experimental procedure

Source type	ROTATING ANODE
Temperature [K]	290
Detector technology	AREA DETECTOR
Collection date	1993
Detector	SIEMENS-NICOLET X100
Spacegroup name	P 32 2 1
Unit cell lengths	64.609, 64.609, 65.191
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 1.800
R-factor	0.167 *
Rwork	0.167
RMSD bond length	0.011
RMSD bond angle	0.030
Data reduction software	XDS
Data scaling software	XDS
Phasing software	X-PLOR
Refinement software	PROFFT

Data quality characteristics

	Overall
Low resolution limit [Å]	28.900
High resolution limit [Å]	1.800
Rmerge	0.034
Number of reflections	13874
<I/σ(I)>	20.17
Completeness [%]	92.6
Redundancy	2.89

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.5		CRYSTALLIZED FROM 80% AMMONIUM SULFATE, pH 5.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	50 (mg/ml)
2	1	reservoir	ammonium sulfate	30 (%)
3	1	reservoir		3.0 (M)
4	1	reservoir	sodium acetate	0.1 (M)