1RNR
AUTOCATALYTIC GENERATION OF DOPA IN THE ENGINEERED PROTEIN R2 F208Y FROM ESCHERICHIA COLI RIBONUCLEOTIDE REDUCTASE AND CRYSTAL STRUCTURE OF THE DOPA-208 PROTEIN
Experimental procedure
Spacegroup name | P 21 21 21 |
Unit cell lengths | 74.100, 85.300, 115.700 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.500 |
R-factor | 0.186 |
Rwork | 0.186 |
RMSD bond length | 0.014 |
RMSD bond angle | 2.800 |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 60.000 * |
High resolution limit [Å] | 2.500 * |
Rmerge | 0.090 * |
Total number of observations | 67726 * |
Number of reflections | 24658 * |
Completeness [%] | 94.5 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6 * | 20 * | Nordlund, P. (1989) FEBS Lett., 258, 251. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG4000 | 20 (%) | |
2 | 1 | reservoir | 0.2 (M) | ||
3 | 1 | reservoir | dioxane | 3 (%) | |
4 | 1 | reservoir | MES | 0.05 (M) | |
5 | 1 | reservoir | EMTS |