1RNF

X-RAY CRYSTAL STRUCTURE OF UNLIGANDED HUMAN RIBONUCLEASE 4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	32.910, 94.270, 40.750
Unit cell angles	90.00, 106.97, 90.00

Refinement procedure

Resolution	8.000 - 2.100
R-factor	0.174
Rwork	0.174
R-free	0.23600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2rat
RMSD bond length	0.012
RMSD bond angle	1.601
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.099
Total number of observations	57662 *
Number of reflections	13467 *
<I/σ(I)>	8.2	2.56
Completeness [%]	97.7	94.8
Redundancy	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.9		30% PEG4000, 0.1 M SODIUM CADODYLATE, PH 6.3, pH 6.9

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	PEG4000	15 (%(v/v))
3	1	reservoir	sodium acetate	0.1 (M)