1RNB

CRYSTAL STRUCTURE OF A BARNASE-D(*GP*C) COMPLEX AT 1.9 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 31 2 1
Unit cell lengths	57.970, 57.970, 85.370
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	5.500 * - 1.900
R-factor	0.214 *
Rwork	0.214
RMSD bond length	0.019
RMSD bond angle	0.061 *
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	1.900 *
Rmerge	0.058 *
Total number of observations	26792 *
Number of reflections	12204 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8 *	20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	ammonium sulfate	3.0 (M)
2	1	reservoir	Barnase	0.7 (mM)
3	1	reservoir	d(GpC)	3.5 (mM)
4	1	reservoir	ammonium sulphate	1.5 (M)