1RN4

HIS92ALA MUTATION IN RIBONUCLEASE T1 INDUCES SEGMENTAL FLEXIBILITY. AN X-RAY STUDY

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	32.760, 55.130, 43.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.800
R-factor	0.148
RMSD bond length	0.016
RMSD bond angle	0.060
Refinement software	PROFFT

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]		1.800 *
Rmerge	0.027 *
Total number of observations	9877 *
Number of reflections	1381 *
Completeness [%]		80 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	1 (%)
2	1	drop	Tris-HCl	20 (mM)
3	1	drop	EDTA	1 (mM)
4	1	drop	PEG4000	30 (%(w/v))