1RMR
Crystal Structure of Schistatin, a Disintegrin Homodimer from saw-scaled Viper (Echis carinatus) at 2.5 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-10-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.541 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 91.645, 91.645, 55.049 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.19216 |
| Rwork | 0.190 |
| R-free | 0.22974 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.333 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 3943 | |
| <I/σ(I)> | 17.8 | 2.7 |
| Completeness [%] | 55 | |
| Redundancy | 10.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | ammonium sulfate, cacodylate, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
