1RMG

RHAMNOGALACTURONASE A FROM ASPERGILLUS ACULEATUS

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1995-07
Detector	RIGAKU
Spacegroup name	I 2 2 2
Unit cell lengths	62.900, 125.400, 137.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.000
R-factor	0.174
Rwork	0.174
R-free	0.21400
Structure solution method	SIRAS
RMSD bond length	0.009
RMSD bond angle	26.700 *
Data reduction software	DENZO
Data scaling software	CCP4
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.061	0.297 *
Number of reflections	34076
<I/σ(I)>	11	3
Completeness [%]	92.7	76.7 *
Redundancy	5.6	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	3.5		Petersen, T.N., (1997) Acta Crystallogr.,Sect.D, 53, 105. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	10 (%)
2	1	reservoir	potassium phosphate	0.05 (M)
3	1	reservoir	sodium acetate	0.1 (M)