1RM9

Probing the Role of Tryptophans in Aequorea Victoria Green Fluorescent Proteins with an Expanded Genetic Code

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	CCD
Collection date	2003-03-20
Detector	MARRESEARCH
Wavelength(s)	1.050
Spacegroup name	P 21 21 21
Unit cell lengths	51.057, 62.507, 69.466
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.900
R-factor	0.2071
Rwork	0.207
R-free	0.26800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1emg
RMSD bond length	0.009
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.940
High resolution limit [Å]	2.900	2.900
Number of reflections	5068
Completeness [%]	96.5	99.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	293	22% PEG 10000, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K