1RLV

Crystal structure of a dimeric Archaeal Splicing Endonuclease

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	1999-07-19
Detector	ADSC
Wavelength(s)	0.9795, 0.9793, 0.9998, 0.9686
Spacegroup name	P 43 21 2
Unit cell lengths	126.817, 126.817, 130.231
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 * - 2.800 *
R-factor	0.229
Rwork	0.229
R-free	0.27700 *
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.025 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *
High resolution limit [Å]	3.000
Rmerge	0.069 *	0.189 *
Total number of observations	291694 *
Number of reflections	21746
Completeness [%]	100.0 *	100 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6.5	298	Pipes, KCL, ammonium sulfate, pH 6.5, EVAPORATION, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PIPES	100 (mM)	pH6.5
3	1	reservoir	ammonium sulfate	25-40 (mM)
4	1	reservoir		120-200 (mM)