1RLM

Crystal Structure of ybiV from Escherichia coli K12

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2002-06-09
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97872,0.97890,0.96373,1.000
Spacegroup name	P 21 21 21
Unit cell lengths	71.823, 91.605, 186.563
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.960 - 1.900
R-factor	0.219
Rwork	0.219
R-free	0.24800
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.600
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.930
High resolution limit [Å]	1.900	1.900
Number of reflections	96340
<I/σ(I)>	23.5	4.5
Completeness [%]	98.6	98.8
Redundancy	4.3	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.3	298	PEG 8000, sodium acetate, cacodylic acid, pH 5.3, VAPOR DIFFUSION, HANGING DROP, temperature 298K