1RLI
The Structure of Trp Repressor Binding Protein from Bacillus subtilis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-07-07 |
Detector | SBC-2 |
Wavelength(s) | 1.07205 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.993, 90.666, 63.446 |
Unit cell angles | 90.00, 97.02, 90.00 |
Refinement procedure
Resolution | 36.790 - 1.800 |
R-factor | 0.199 |
Rwork | 0.196 |
R-free | 0.23000 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.400 |
Data reduction software | d*TREK |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.790 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.071 | 0.528 |
Number of reflections | 62699 | |
<I/σ(I)> | 9.3 | 1.85 |
Completeness [%] | 98.8 | 95.3 |
Redundancy | 4.2 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | sodium, potassium phosphate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |