1RLI
The Structure of Trp Repressor Binding Protein from Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-07 |
| Detector | SBC-2 |
| Wavelength(s) | 1.07205 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.993, 90.666, 63.446 |
| Unit cell angles | 90.00, 97.02, 90.00 |
Refinement procedure
| Resolution | 36.790 - 1.800 |
| R-factor | 0.199 |
| Rwork | 0.196 |
| R-free | 0.23000 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data reduction software | d*TREK |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.790 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.071 | 0.528 |
| Number of reflections | 62699 | |
| <I/σ(I)> | 9.3 | 1.85 |
| Completeness [%] | 98.8 | 95.3 |
| Redundancy | 4.2 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | sodium, potassium phosphate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
