1RLH

Structure of a conserved protein from Thermoplasma acidophilum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	150
Detector technology	CCD
Collection date	2002-07-15
Detector	CUSTOM-MADE
Wavelength(s)	1.03320
Spacegroup name	P 63
Unit cell lengths	74.329, 74.329, 50.840
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.900 - 1.800
R-factor	0.196
Rwork	0.179
R-free	0.20800
Structure solution method	MAD + Molecular replacement
RMSD bond length	0.004
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	d*TREK
Phasing software	SHARP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	39.900
High resolution limit [Å]	1.750
Number of reflections	14981

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	294	sodium acetate, NaCl, PEG 3350, glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 294K
1	VAPOR DIFFUSION, HANGING DROP	5	294	sodium acetate, NaCl, PEG 3350, glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 294K
1	VAPOR DIFFUSION, HANGING DROP	5	294	sodium acetate, NaCl, PEG 3350, glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 294K