1RLA
THREE-DIMENSIONAL STRUCTURE OF RAT LIVER ARGINASE, THE BINUCLEAR MANGANESE METALLOENZYME OF THE UREA CYCLE
Experimental procedure
Detector technology | IMAGE PLATE |
Collection date | 1994-09-17 |
Detector | RIGAKU |
Spacegroup name | P 32 |
Unit cell lengths | 88.500, 88.500, 106.200 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.100 |
R-factor | 0.178 |
Rwork | 0.178 |
R-free | 0.22900 |
RMSD bond length | 0.011 * |
RMSD bond angle | 1.600 * |
Data reduction software | MOSFLM |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
High resolution limit [Å] | 2.100 * | |
Rmerge | 0.056 | 0.365 * |
Total number of observations | 147454 * | |
Number of reflections | 46162 * | |
Completeness [%] | 91.6 * | 58.3 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8.5 * | 4 * | Kanyo, Z.F., (1992) J.Mol.Biol., 224, 1175. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 16 (mg/ml) | |
2 | 1 | drop | Bicine | 50 (mM) | |
3 | 1 | drop | 1 (mM) | ||
4 | 1 | reservoir | PEG8000 | 14 (%(w/v)) | |
5 | 1 | reservoir | Bicine | 50 (mM) | |
6 | 1 | reservoir | 1 (mM) | ||
7 | 1 | reservoir | sodium azide | 0.05 (%(w/v)) |