1RL6
RIBOSOMAL PROTEIN L6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 71.800, 71.800, 124.500 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.000 |
R-factor | 0.257 * |
Rwork | 0.257 |
R-free | 0.30200 |
Structure solution method | MIR |
RMSD bond length | 0.014 |
RMSD bond angle | 1.600 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASES |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.090 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.080 | |
Number of reflections | 13166 | |
Completeness [%] | 97.5 | 97.4 |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.6 | pH 7.6 |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | NA/K PHOSPHATE | ||
2 | 1 | 1 | BETA MERCAPTOETHANOL | ||
3 | 1 | 1 | 1,4-DIOXANE |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10-15 (mg/ml) | |
2 | 1 | drop | sodium potassium phosphate | 0.6 (M) | |
3 | 1 | drop | beta-mercaptoethanol | 3 (mM) | |
4 | 1 | drop | 1,4-dioxane | 3 (%(v/v)) | |
5 | 1 | reservoir | sodium potassium phospahte | 1.6-1.8 (M) | |
6 | 1 | reservoir | beta-mercaptoethanol | 3 (mM) | |
7 | 1 | reservoir | 1,4-dioxane | 3 (%) |