1RKQ
Crystal structure of HAD-like phosphatase yidA from E. coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X9A |
Synchrotron site | NSLS |
Beamline | X9A |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 2003-08-06 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97901 |
Spacegroup name | P 1 |
Unit cell lengths | 45.120, 54.871, 67.725 |
Unit cell angles | 112.58, 96.37, 106.79 |
Refinement procedure
Resolution | 24.500 - 1.400 |
Rwork | 0.177 |
R-free | 0.21300 |
Structure solution method | Single Crystal SIRAS by RIP |
Starting model (for MR) | Experimental Phasing by single crystal SIRAS (Radiation damage). |
RMSD bond length | 0.020 |
RMSD bond angle | 0.034 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | RESOLVE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.000 | 1.450 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.043 | 0.370 |
Number of reflections | 106331 | |
<I/σ(I)> | 30.9 | 2.58 |
Completeness [%] | 95.9 | 93.4 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | PEG 3350, Magnesium Chloride,, VAPOR DIFFUSION, HANGING DROP, temperature 277K | |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | PEG 3350, Magnesium Chloride,, VAPOR DIFFUSION, HANGING DROP, temperature 277K |