1RK3
crystal structure of the rat vitamin D receptor ligand binding domain complexed with 1,25-dihydroxyvitamin D3 and a synthetic peptide containing the NR2 box of DRIP 205
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-10-24 |
| Detector | BRUKER PROTEUM R |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 154.726, 44.154, 42.072 |
| Unit cell angles | 90.00, 96.13, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.200 |
| R-factor | 0.19303 |
| Rwork | 0.190 |
| R-free | 0.24255 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1rjk |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.384 |
| Data reduction software | SAINT |
| Data scaling software | LSCALE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.440 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 14475 | |
| <I/σ(I)> | 10.9 | 2.7 |
| Completeness [%] | 99.0 | 95 |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | PEG 4000, MOPS, ammonium citrate, isopropanol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
