1RJU
Crystal structure of a truncated form of yeast copper thionein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.91920 |
| Spacegroup name | P 43 3 2 |
| Unit cell lengths | 62.167, 62.167, 62.167 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.840 - 1.440 |
| R-factor | 0.14687 |
| Rwork | 0.145 |
| R-free | 0.17072 |
| Structure solution method | SAD |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.073 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.840 | 1.520 |
| High resolution limit [Å] | 1.440 | 1.440 |
| Rmerge | 0.070 | 0.358 |
| Number of reflections | 7922 | |
| <I/σ(I)> | 9.3 | 2.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 21.8 | 22.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 293 | 250 mM KH2PO4, 1mM LiBr, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
