1RJF
Structure of PPM1, a leucine carboxy methyltransferase involved in the regulation of protein phosphatase 2A activity
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-02-14 |
Detector | MARRESEARCH |
Wavelength(s) | 0.98000 |
Spacegroup name | P 65 |
Unit cell lengths | 112.395, 112.395, 162.848 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.250 |
R-factor | 0.1857 |
Rwork | 0.183 |
R-free | 0.23900 * |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.800 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.370 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.070 * | |
Total number of observations | 290033 * | |
Number of reflections | 53908 | |
Completeness [%] | 97.6 | 86 |
Redundancy | 5.4 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 15% PEG 8000, 0.1M K/NaPO4, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 3 (mg/ml) |