1RJ7

Crystal structure of EDA-A1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-06-25
Detector	MARRESEARCH
Wavelength(s)	1.08
Spacegroup name	P 1 21 1
Unit cell lengths	53.657, 279.687, 54.175
Unit cell angles	90.00, 91.36, 90.00

Refinement procedure

Resolution	20.000 - 2.300
R-factor	0.20127
Rwork	0.194
R-free	0.26100 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	truncated model of Apo2L/TRAIL from 1d0g
RMSD bond length	0.010
RMSD bond angle	1.280 *
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.1.07)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.081	0.239 *
Total number of observations	215586 *
Number of reflections	66596 *
<I/σ(I)>	8.6	3
Completeness [%]	94.4 *	93.1 *
Redundancy	3.2	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	19 *	20% PEG 3350, 0.2M di-sodium hydrogen phosphate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	9.5 (mg/ml)
2	1	reservoir	PEG3350	20 (%)
3	1	reservoir	di-sodium hydrogen phosphate	0.2 (M)