1RJ5
Crystal Structure of the Extracellular Domain of Murine Carbonic Anhydrase XIV
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-07-11 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.100 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 59.000, 75.600, 73.200 |
Unit cell angles | 90.00, 98.90, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.800 * |
R-factor | 0.234 |
Rwork | 0.234 |
R-free | 0.27400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jcz |
RMSD bond length | 0.006 |
RMSD bond angle | 25.200 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.099 * | 0.312 * |
Number of reflections | 14643 | |
<I/σ(I)> | 8.3 | 2.5 |
Completeness [%] | 93.8 | 94.3 |
Redundancy | 3 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 * | 294 | PEG 4000, sodium acetate, sodium chloride, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | enzyme | 7 (mg/ml) | |
2 | 1 | drop | sodium phosphate | 20 (mM) | pH7.2 |
3 | 1 | drop | 150 (mM) | ||
4 | 1 | reservoir | PEG4000 | 5.5 (%(w/v)) | |
5 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.8 |
6 | 1 | reservoir | 20 (mM) |