1RIT
Crystal structure of Peanut lectin in complex with meso-tetrasulphonatophenylporphyrin and lactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-10-08 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 |
| Unit cell lengths | 94.900, 94.900, 144.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 100.000 - 2.850 |
| R-factor | 0.235 |
| Rwork | 0.235 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.950 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.091 | 0.761 |
| Number of reflections | 33919 | |
| <I/σ(I)> | 5.9 | |
| Completeness [%] | 87.8 | 86.5 |
| Redundancy | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 298 | Ammonium sulphate, Sodium Chloride, Phosphate buffe, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
