1RIB

STRUCTURE AND FUNCTION OF THE ESCHERICHIA COLI RIBONUCLEOTIDE REDUCTASE PROTEIN R2

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	74.300, 85.500, 115.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.000 - 2.200
R-factor	0.175
Rwork	0.175
RMSD bond length	0.011
RMSD bond angle	2.840
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.200 *
Rmerge	0.059 *
Total number of observations	90054 *
Number of reflections	27364 *
Completeness [%]	74.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6 *	20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	MES	0.05 (M)
2	1	reservoir	PEG4000	20 (%(v/v))
3	1	reservoir		0.2 (M)
4	1	reservoir	dioxane	0.3 (%(v/v))
5	1	reservoir	ethylmercurithiosalicylate	0.05 (M)