1RHL
RIBONUCLEASE T1 COMPLEXED WITH 2'GMP/G23A MUTANT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-05-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.440, 48.230, 50.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.950 |
| R-factor | 0.164 |
| Rwork | 0.164 |
| R-free | 0.20800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rga |
| RMSD bond length | 0.006 |
| RMSD bond angle | 24.930 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.040 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.126 | 0.475 |
| Total number of observations | 50578 * | |
| Number of reflections | 7378 | |
| <I/σ(I)> | 10.7 | 3.3 |
| Completeness [%] | 96.2 | 94.1 |
| Redundancy | 6.84 | 6.75 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.2 | used to seeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | 2'-GMP | 2 (mM) | |
| 3 | 1 | reservoir | 2 (mM) | ||
| 4 | 1 | reservoir | MPD | 50 (%) | pH4.2 |
