1RHF
Crystal Structure of human Tyro3-D1D2
Experimental procedure
| Experimental method | MAD |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-05-07 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 0.9766, 0.9762, 0.9252 |
| Spacegroup name | P 61 |
| Unit cell lengths | 109.550, 109.550, 62.470 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.960 |
| R-factor | 0.21286 |
| Rwork | 0.209 |
| R-free | 0.25600 * |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.402 |
| Data scaling software | XDS |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.277 * | |
| Total number of observations | 150721 * | |
| Number of reflections | 30568 | |
| Completeness [%] | 0.0 * | 82.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 20 * | HEPES, sodium cacodylate, magnesium acetate, PEG2000, DTT, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 17 (mg/ml) | |
| 2 | 1 | drop | HEPES | 20 (mM) | pH7.0 |
| 3 | 1 | drop | sodium cacodylate | 100 (mM) | pH5.4-5.7 |
| 4 | 1 | drop | PEG2000 | 10-14 (%) | |
| 5 | 1 | drop | magnesium acetate | 200 (mM) | |
| 6 | 1 | drop | dithiothreitol | 4 (mM) | |
| 7 | 1 | reservoir | sodium cacodylate | 100 (mM) | pH5.5 |
| 8 | 1 | reservoir | PEG2000 | 12 (%) | |
| 9 | 1 | reservoir | magnesium acetate | 200 (mM) |
