1RHC
F420-dependent secondary alcohol dehydrogenase in complex with an F420-acetone adduct
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Temperature [K] | 277 |
Detector technology | CCD |
Collection date | 2003-01-22 |
Detector | MARRESEARCH |
Wavelength(s) | 1.04 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 86.824, 156.316, 60.199 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.760 - 1.800 |
R-factor | 0.158 |
Rwork | 0.158 |
R-free | 0.18800 |
Structure solution method | MIR |
RMSD bond length | 0.013 |
RMSD bond angle | 1.600 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXD |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.000 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 45082 | |
<I/σ(I)> | 21 | 3.9 |
Completeness [%] | 98.9 | 99.6 |
Redundancy | 3.8 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 277 | PEG 8000, sodium chloride, sodium citrate, pH 4, VAPOR DIFFUSION, HANGING DROP, temperature 277K |