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1RH7

Crystal Structure of Resistin-like beta

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsNSLS BEAMLINE X4A
Synchrotron siteNSLS
BeamlineX4A
Temperature [K]110
Detector technologyCCD
Collection date2002-06-14
DetectorADSC QUANTUM 4
Wavelength(s)1.06975
Spacegroup nameI 2 2 2
Unit cell lengths57.604, 86.016, 283.406
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution14.960 - 3.106
R-factor0.20431
Rwork0.201
R-free0.26380
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1rgx
RMSD bond length0.011
RMSD bond angle1.557
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareAMoRE
Refinement softwareREFMAC (5.1)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]15.0003.210
High resolution limit [Å]3.1003.100
Rmerge0.1450.325
Number of reflections13231
<I/σ(I)>209.5
Completeness [%]100.0100
Redundancy14.2
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP6.52772.5M NaCl, 0.1M Bis-Tris pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K

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