1RH6
Bacteriophage Lambda Excisionase (Xis)-DNA Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-03-07 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.10 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 80.215, 72.691, 38.801 |
| Unit cell angles | 90.00, 104.11, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.19293 |
| Rwork | 0.191 |
| R-free | 0.22553 |
| Structure solution method | MIR |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.582 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.780 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.050 | 0.135 |
| Number of reflections | 32190 | |
| <I/σ(I)> | 19.8 | 8.2 |
| Completeness [%] | 93.1 | 95.8 |
| Redundancy | 93.1 | 95.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | NaOAc, imidazole, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | NaOAc | ||
| 2 | 1 | 1 | imidazole | ||
| 3 | 1 | 1 | H2O | ||
| 4 | 1 | 2 | NaOAc | ||
| 5 | 1 | 2 | imidazole | ||
| 6 | 1 | 2 | H2O |
