1RH5

The structure of a protein conducting channel

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 8-BM
Synchrotron site	APS
Beamline	8-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2003-03-01
Detector	MARRESEARCH
Wavelength(s)	0.98
Spacegroup name	P 21 21 2
Unit cell lengths	102.930, 156.650, 69.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 3.200
R-factor	0.242
Rwork	0.242
R-free	0.28700 *
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.230 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	3.310
High resolution limit [Å]	3.200	3.200
Rmerge	0.050 *	0.440 *
Number of reflections	17396	1439 *
<I/σ(I)>	26.9	2.24
Completeness [%]	93.2	76.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.5	4 *	Peg 400, Glycine buffer, glycerol, sodium chloride, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5-10 (mg/ml)
2	1	reservoir	PEG400	50-55 (%)
3	1	reservoir	glycine	50 (mM)	pH9.0-9.5