1RH4
RH4 DESIGNED RIGHT-HANDED COILED COIL TETRAMER
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 300 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-06 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 37.556, 37.556, 53.384 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 1.900 |
| R-factor | 0.2 |
| Rwork | 0.200 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PREDICTED STRUCTURE |
| RMSD bond length | 0.007 |
| RMSD bond angle | 14.460 * |
| Data reduction software | R-AXIS |
| Data scaling software | R-AXIS |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 6.000 | 1.980 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.034 | |
| Number of reflections | 3175 | |
| Completeness [%] | 86.0 | 58.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8.1 | 100MM TRIS PH 8.1 0.3M NABR 20% 2-PROPANOL 20% POLYETHLENE GLYCOL 1450 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 2 | 1 | reservoir | 0.3 (M) | ||
| 3 | 1 | reservoir | isopropanol | 20 (%) | |
| 4 | 1 | reservoir | PEG1450 | 20 (%) |
