1RGX
Crystal Structure of resisitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2000-12-02 |
| Detector | MACSCIENCE |
| Wavelength(s) | 1.0542517 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 49.021, 52.475, 96.609 |
| Unit cell angles | 90.00, 96.53, 90.00 |
Refinement procedure
| Resolution | 17.820 - 1.787 |
| R-factor | 0.20577 |
| Rwork | 0.204 |
| R-free | 0.24494 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rfx |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.844 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.860 |
| High resolution limit [Å] | 1.787 | 1.787 |
| Rmerge | 0.083 | 0.249 |
| Number of reflections | 21591 | |
| <I/σ(I)> | 11.3 | 4.6 |
| Completeness [%] | 98.4 | 100 |
| Redundancy | 17.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | 40% MPD, 8% PEG 5000MME, 0.2M MgCl2, 0.1M Na Acetate pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
