1RGL

RNASE T1 MUTANT GLU46GLN BINDS THE INHIBITORS 2'GMP AND 2'AMP AT THE 3' SUBSITE

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	49.160, 46.760, 41.090
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	? - 2.000
R-factor	0.148
RMSD bond length	0.010
RMSD bond angle	2.033
Refinement software	TNT

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000 *
High resolution limit [Å]	2.000 *
Rmerge	0.030 *
Total number of observations	11009 *
Number of reflections	5721 *
Completeness [%]	85.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	E46Q	1 (mM)
2	1	1	2'GMP	13.5 (mM)
3	1	2	MPD	50 (%(v/v))
4	1	2	2'GMP	1 (mM)