1RGI
Crystal structure of gelsolin domains G1-G3 bound to actin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2002-11-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0052 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 145.245, 145.245, 129.948 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.960 - 3.000 |
| R-factor | 0.22579 |
| Rwork | 0.224 |
| R-free | 0.25755 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p8z |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.393 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 31104 | |
| <I/σ(I)> | 21.3 | 3.2 |
| Completeness [%] | 98.5 | |
| Redundancy | 2.9 | 3.87 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 277 | 2% PEG 8000, 100 mM Sodium acetate, 1 mM CaCl2, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
