1RGE

HYDROLASE, GUANYLORIBONUCLEASE

Experimental procedure

Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X31
Synchrotron site	EMBL/DESY, Hamburg
Beamline	X31
Detector technology	IMAGE PLATE
Collection date	1993-11
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	64.730, 78.560, 38.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.150
RMSD bond angle	0.019
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXL-93
Refinement software	SHELXL-93

Data quality characteristics

	Overall
High resolution limit [Å]	1.150 *
Rmerge	0.066
Total number of observations	445145 *
Number of reflections	62845 *
Completeness [%]	87.3 *
Redundancy	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.7		ROOM TEMPERATURE, PH 6.7, AMMONIUM SULFATE

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	3.0 (%)
2	1	drop	phosphate	0.1 (M)
3	1	reservoir	ammonium sulfate	22 (%sat)